Blar i forfatter "Nielsen, Jeppe Lund"

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    • eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants 

      Nørgaard, Louise; Olesen, Carsten Riis; Trøjelsgaard, Kristian; Pertoldi, Cino; Nielsen, Jeppe Lund; Taberlet, Pierre Robert Michel; Ruiz-Gonzalez, Aritz; De Barba, M.; Iacolina, Laura (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-03-25)
      With an accelerating negative impact of anthropogenic actions on natural ecosystems, non-invasive biodiversity assessments are becoming increasingly crucial. As a consequence, the interest in the application of environmental DNA (eDNA) survey techniques has increased. The use of eDNA extracted from faeces from generalist predators, have recently been described as “biodiversity capsules” and suggested ...

    • Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from low-biomass clay samples 

      Jacobsen, Carsten Suhr; Nielsen, Tue Kjærgaard; Vester, Jan Kjølhede; Stougaard, Peter; Nielsen, Jeppe Lund; Voriskova, Jana; Winding, Anne; Baldrian, Petr; Liu, Binbin; Frostegård, Åsa; Pedersen, Dorthe; Tveit, Alexander Tøsdal; Svenning, Mette Marianne; Tebbe, Christoph C.; Øvreås, Lise; Jakobsen, Pia Bach; Blazewicz, Steven J.; Hubablek, Valerie; Bertilsson, Stefan; Hansen, Lars Hestbjerg; Cary, S. Craig; Holben, William E.; Ekelund, Flemming; Bælum, Jacob (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-04-09)
      Here we show that a commercial blocking reagent (G2) based on modified eukaryotic DNA significantly improved DNA extraction efficiency. We subjected G2 to an inter-laboratory testing, where DNA was extracted from the same clay subsoil using the same batch of kits. The inter-laboratory extraction campaign revealed large variation among the participating laboratories, but the reagent increased the ...